31Oct/24

KMD Bioscience-Cas9 Gene Editing

10/31/2024CRO ServiceCas9 Gene Editingkmdbioscience

Cas9 gene editing, commonly referred to as CRISPR-Cas9 gene editing, is a revolutionary technology that enables precise modifications of DNA in living organisms. It is based on a natural defense mechanism found

30Oct/24

KMD Bioscience-Western Blot Steps

10/30/2024Protein EngineeringWestern Blot Stepskmdbioscience

Here is a detailed breakdown of the steps involved in performing a Western blot: Western Blot Steps Sample Preparation Lysis of Cells or Tissues: Lyse the cells or tissues to extract proteins

29Oct/24

KMD Bioscience-Flow Cytometry Assay

10/29/2024CRO ServiceFlow Cytometry Assaykmdbioscience

A flow cytometry assay is a method used to measure various physical and chemical characteristics of cells or particles using flow cytometry. Flow cytometry assays are highly versatile and allow for the

24Oct/24

KMD Bioscience-Flow Cytometry Analysis

10/24/2024CRO ServiceFlow Cytometry Analysiskmdbioscience

Flow cytometry analysis is a powerful technique used to analyze the physical and chemical characteristics of cells or particles as they flow in a fluid stream through a laser beam. This technique

23Oct/24

KMD Bioscience-ELISA screening

10/23/2024Antibody DiscoveryELISA screeningkmdbioscience

ELISA screening refers to using the Enzyme-Linked Immunosorbent Assay (ELISA) as a high-throughput method to screen a large number of samples for the presence of a specific antigen or antibody. This approach

22Oct/24

KMD Bioscience-Elisa Assay Development

10/22/2024CRO ServiceElisa Assay Developmentkmdbioscience

Enzyme-Linked Immunosorbent Assay (ELISA) development involves several critical steps to create a sensitive, specific, and reproducible assay for detecting proteins, peptides, antibodies, or hormones in biological samples. The process of developing a

17Oct/24

KMD Bioscience-Yeast One-hybrid Assay

10/17/2024Protein EngineeringYeast One-hybrid Assaykmdbioscience

The Yeast One-Hybrid (Y1H) Assay is a molecular biology technique used to identify protein-DNA interactions. While the Yeast Two-Hybrid (Y2H) assay focuses on protein-protein interactions, the Y1H assay is specifically designed to

16Oct/24

KMD Bioscience-Protein Assay Protocol

10/16/2024Protein EngineeringProtein Assay Protocolkmdbioscience

Protein assays are used to measure the concentration of proteins in a sample, which is essential for downstream applications such as enzyme assays, Western blotting, and protein purification. Several protein assays exist,

15Oct/24

KMD Bioscience-Plasmid Construction Steps

10/15/2024Protein EngineeringPlasmid Construction Stepskmdbioscience

Plasmid construction involves designing and assembling a recombinant DNA molecule that can be propagated in a host organism (such as bacteria, yeast, or mammalian cells) to express a gene of interest or

12Oct/24

KMD Bioscience-Mammalian Protein Expression System

10/12/2024Protein EngineeringMammalian Protein Expression Systemkmdbioscience

The mammalian protein expression system is widely used for producing recombinant proteins that require proper folding, post-translational modifications (PTMs), and biological activity, similar to native proteins in human cells. Mammalian systems are

Month: October 2024